Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4 / 生物工程学报

We isolated and identified the symbiotic and adnascent microorganisms from an unidentified sponge collected from 10-meter-deep seawater of the Paracel Islands in China. A total of 16 strains were obtained and identified. Through bacteriostatic activity assay, one of the strains, Dermacoccus sp. X4, was found to effectively inhibit the growth of Staphylococcus aureus. Subsequently, its secondary metabolites were purified by silica gel partition, octadecylsilane (ODS) reverse phase, Sephadex™LH-20 size exclusion, and C18 reverse phase chromatography. Using liquid chromatography, mass spectrometry, and nuclear magnetic resonance, three of the purified compounds were structurally characterized to be one 3-(4-hydroxybenzyl) hexahydropyrrolo [1,2-a]pyrazine-1,4-dione and two indole acid glycerides. This is the first report about indole acid glyceride isolated from microbial secondary metabolites, enriching marine drug candidate resources.

Potential of Co-culture Induction in Antibiotics Discovery from Bacteria-associated with Marine Sponge Hymeniacidon perleve / 微生物学通报

Ten actinomycetes strains from inter-tidal marine sponge Hymeniacidon perleve of China Yellow Sea were isolated and bio-assayed for antibiotic activities.3 of the 10 strains displayed antimicrobial activity when in monoculture.While strain Hp-053 was co-cultured with each of the other 9 strains,5 of the 9 co-cultures exhibited enhancing antimicrobial activity.TLC results and bio-autography of extracted samples indicated that chemical compounds and antimicrobial compounds produced by co-cultures of sponge associated actinomycetes were different from those by monoculture.Co-culture induction may be a valuable novel tool for the exploitation of sponge associated microorganism.